中国水稻科学2011，Vol.25Issue(1)：19-24,6.DOI:10.3969/j.issn.1001-7216.2011.01.003
2个杂交籼稻和2个粳稻品种SSR指纹图谱的构建及双重PCR技术的初步研究
Construction of Molecular Fingerprinting Database and Dual PCR Assay for Two Indica Rice Hybrids and Two Japonica Rice Varieties by Using SSR Markers
摘要
Abstract
A total of 460 SSR primers were used for amplification of five indica hybrid rice combinations and their parents, and 16 japonica rice varieties (or elite lines). By using the identified co-dominant specific primers, molecular fingerprinting databases for Guodao 6, Liangyoupeijiu, Yandao 8 and Xudao 4 were constructed. A total of 5, 3, 1 and 2 SSR primers were identified to be specific for Guodao 6, Liangyoupeijiu, Yandao 8 and Xudao 4, respectively. These fingerprints can be used to examine the seed purity of these rice varieties. In addition, to develop dual PCR assay in examination of seed purity and authenticity, SSR primers which could stably amplify clear bands and have good specificity were selected and used in multiplex popymerase chain reaction amplification.关键词
杂交水稻/种子纯度/种子真实性/指纹图谱/双重聚合酶链式反应/种子鉴定分类
农业科技引用本文复制引用
陈树林,王海燕,丁震乾,王秀娥,陈忠明..2个杂交籼稻和2个粳稻品种SSR指纹图谱的构建及双重PCR技术的初步研究 [J].中国水稻科学,2011,25(1):19-24,6.基金项目
江苏省成果转化专项资金资助项目(DA2005008). （DA2005008）
