中国水稻科学2011，Vol.25Issue(1)：25-30,6.DOI:10.3969/j.issn.1001-7216.2011.01.004
抗稻瘟病基因Pi9的STS连锁标记开发及在分子标记辅助育种中的应用
Development of STS Marker Linked to Rice Blast Resistance Gene Pi9 in Marker-Assisted Selection Breeding
摘要
Abstract
Indica rice line 75-1-127 carrying broad-spectrum rice blast resistance gene Pi9 was used as resistance gene donor for genetic improvement of Yangdao 6 and R6547.By alignment of genomic sequences from the Pi9 locus of 75-1-127, Yangdao 6 and Nipponbare, co-dominant STS (sequence-tagged site) marker PB9-1, which linked to the Pi9 gene, was developed for molecular marker-assisted selection (MAS).Eight backcross lines carrying Pi9 were obtained, among which four lines contained the genetic background of Yangdao 6 and the other four lines had the R6547 background.Under natural disease infection conditions in Enshi County and Yichang County of Hubei Province, China, the Pi9-carrying lines showed higher blast disease resistance than Yangdao 6 and R6547 in various degrees.The hybrids originated from 08C893, an R6547-derived rice line, showed higher blast disease resistance as compared to the control Yangliangyou 6.The results suggest PB9-1 is an effective codominant marker for MAS of Pi9 which is a valuable resistance gene for the control of rice blast disease in Hubei area.关键词
稻瘟病抗性/分子标记辅助选择/序列标记位点/抗性基因/育种/水稻分类
农业科技引用本文复制引用
殷得所,夏明元,李进波,万丙良,査中萍,杜雪树,戚华雄..抗稻瘟病基因Pi9的STS连锁标记开发及在分子标记辅助育种中的应用 [J].中国水稻科学,2011,25(1):25-30,6.基金项目
湖北省攻关重大专项资助项目(2006AA201A03) （2006AA201A03）
国家863计划资助项目(2006AA100101) （2006AA100101）
国家科技支撑计划资助项目(2006BAD01A01). （2006BAD01A01）
