中国水稻科学2011，Vol.25Issue(1)：31-36,6.DOI:10.3969/j.issn.1001-7216.2011.01.005
利用Wx和fgr基因双功能性标记高效选育优质水稻保持系
Efficient Selection of Fine Rice Quality Maintainer Lines with Functional Markers of Wx and fgr Genes
摘要
Abstract
According to the genotypes detected by two primers of functional markers, 484/485 (for Wx gene) and fgr-1F/1R (for fgr gene), amylose contents and fragrance of 13 indica maintainer lines, Yixiang 1B was selected as good rice quality donor parent. The two markers were used to genotype the F2 individual plants derived from Ⅱ -32B/Yixiang 1B. There were significant difference in electrophoretogram among individual plants, so that they were easy to distinguish, and these two markers showed independent inheritance. Superior individuals with fgr and Wx genotype Ⅰ (same as Yixiang 1B) and Ⅲ (heterozygote)were selected. Then combined with the selection of agronomic and rice quality characters, the detection in selected plants or individual plants in F3 to F6 generations were carried out. In F6 generation, the plants were detected by 484/485 and fgr-1F/1R again, advanced plants were obtained, which had stable characters, good plant type, obvious fragrance, medium amylose content and so on. Results of testcross showed that 75.5％ plants were maintainer lines. It is suggested that detection and selection of maintainer lines of indica rice with functional markers of W.r and fgr genes in early-generation combined with conventional breeding could provide a rapid way to obtain good rice quality maintainer lines with fragrance, medium amylose content,slender grain shape, low chalkiness degree, high transparency and so on.关键词
籼稻/分子标记辅助选择/直链淀粉含量/香味/功能性标记分类
农业科技引用本文复制引用
陈深广,周屹峰,赵霏,金亮,沈圣泉..利用Wx和fgr基因双功能性标记高效选育优质水稻保持系 [J].中国水稻科学,2011,25(1):31-36,6.基金项目
浙江省"8812"计划资助项目(2007C12901) （2007C12901）
浙江省科技攻关计划资助项目(2008C22075) （2008C22075）
农业部行业专项资助项目(200803034). （200803034）
