中国免疫学杂志2011，Vol.27Issue(1)：11-14,4.DOI:10.3969/j.issn.1000-484X.2011.01.003
固相MICA协同s4-1BBL、IL-15体外高效扩增CD3+CD56+细胞的研究
High expansion of CD3 and CD56 double positive cells with immobilized MHC class Ⅰ chain-related protein A,soluble 4-1BBL,and IL-15 in vivo
摘要
Abstract
Objective: To investigate whether immobilized MHC class Ⅰ chain-related protein A (MICA) could synergize with soluble 4-1BBL and IL-15 to promote expansion of peripheral CD3 and CD56 double positive cells ex vivo.Methods: Peripheral blood mononuclear cells or purified CD3 + CD56 + cells were stimulated with immobilized MICA, s4-1BBL, and IL- 15 for 19 days.Frequencies of CD3 + CD56 + cells or the absolute cell numbers were observed during this long-term culture period.Next cytotoxicity and IFN-γ, IL-4 production of the expanded CD3 + CD56 + cells were detected by the LDH releasing assay and ELISA, respectively.Lastly, expression of function-associated receptors on CD3+ CD56+ cells was analyzed.Results:With 19-day culture,frequency of CD3+ CD56+ cells arrived from 2％ to 21.7％ on the average,and CD3+ CD56+ cells conld expand with 32 folds meanly.In addition, the long-term cultured CD3 + CD56+ cells were showed higher cytotoxicity and more production of IFN-γ, and could not secrete IL-4.Activating receptors were up-regulated to be present on the cultured CD3 +CD56+ cells whereas inhibitory receptors were decreased.Conclusion: Immobilized MICA in combination with soluble 4-1BBL and IL-15 not only promotes high expansion of CD3 + CD56 + cells, but also enhances function of CD3 + CD56 + cells.The culture system we developed here may be used in tumor adoptive immunotherapy.关键词
MICA/4-1BBL/IL-15/CD3+CD56+细胞/扩增分类
医药卫生引用本文复制引用
温晓星,葛鹏,龚卫娟,肖炜明,季明春..固相MICA协同s4-1BBL、IL-15体外高效扩增CD3+CD56+细胞的研究[J].中国免疫学杂志,2011,27(1):11-14,4.基金项目
本文为国家自然科学基金(30671917)、江苏省自然科学基金(BK2008215)和江苏省大学生实践创新训练计划资助项目 （30671917）
