摘要
Abstract
Objective: To study the linkage of cytokines and Bβ-854G/A,455G/A,-249C/T,-148C/T, 448G/A, Bcl-1G/A gene polymorphisms with fibrinogen functional features such as plasma fibrinogen concentration and molecular reactivity. Methods: 1 391 subjects of retired employees from Kailuan Group were enrolled in the medical examination and questionnaire survey. C reactive protein (CRP), tumor necrosis factor(TNF-α), interleukin-6( IL-6), IL-8, IL-10 and blood biochemistry index, Fg concen1ration, fibrin monomer polymerized velocity (FMPV) ,absorbance maximum(Amax) and FMPV/Amax were measured in all the individuals. The gene polymorphisms at six sites were detected by polymerase chain reaction-restriction fragnent length polymorphism. Results: IL-6, FMPV/Amax and Fg concentration were significantly different among Bβ-854 genotypes( P ＜0.05).IL-8 was different among BβBcl-1 genotypes( P ＜0.05).The result of multiple stepwise regression analysis showed that IL-6 and IL-8 as dependent variable. Fg concentration and locus gene polymorphisms on Bβ-854,-249, Bcl-1 were selected( P ＜ 0. 05);With IL-10 and TNF-α as dependent variable, FMPV/Amax and locus genes on Bβ448, Bcl-1 were entry( P ＜0. 05 ); FMPV/Amax as dependent variable, Bβ48AA genotype, Bβ-854GA genotype, Fg concentration, IL- 10 and TNF-α were selected in turn ( P ＜ 0.05). Conclusion:Gene regulation,plasma fibrinogen concentration and molecular reactivity in body have correlation with multiple factors and multiple locus gene polymorphisms on Fg beta-chain. It is found that the plasma fibrinogen concentration was significantly associated with the factors of IL-6, IL-8, and locus gene polymorphisms on FgBβ-854,-249 and Bcl-1. The associations of plasma fibrinogen molecular reactivity in body with the cytokine of TNF-α and IL-10, locus gene on FgBβ48 and Bcl-1 were significant on our results. Plasma fibrinegen concentration and molecular reactivity in body can be regulated each other.关键词
纤维蛋白原/多位点基因多态性/细胞因子/基因调控/分子聚合功能分类
医药卫生
