中国水稻科学2011，Vol.25Issue(1)：99-102,4.DOI:10.3969/j.issn.1001-7216.2011.01.016
水稻开花期高温胁迫下的花粉育性QTL定位
Quantitative Trait Loci Associated with Pollen Fertility Under High Temperature Stress at Flowering Stage in Rice
摘要
Abstract
The quantitative trait loci (QTLs) underlying tolerance to high temperature stress(HTS) were identified using the recombinant inbred lines (RILs) derived from a cross between the heat tolerant rice line 996 and the sensitive line 4628. Pollen fertility was used as the heat-tolerance indicator for the lines, which was determined when the lines was subjected to HTS at the flowering stage in field experiments. Two QTLs that affected pollen fertility were detected in the interval between RM5687 and RM471 on chromosome 4, and between RM190 and RM225 on chromosome 6 by using composite interval mapping (CIM)analysis. The QTL qPF4 on chromosome 4 explained 15.1％ of the total variation in pollen fertility, and increased the pollen fertility of HTS-subjected plants by 7.15％. The second QTL qPF6 on chromosome 6 explained 9.31 ％ of the total variation in pollen fertility, and increased the pollen fertility of plants subjected to HTS by 5.25％. The positive additive effects of the two QTLs were derived from the line 996 alleles. The two major QTLs identified should be useful for further fine mapping and cloning of these genes and for molecular marker-assisted selection of heat-tolerant rice cultivars.关键词
水稻/数量性状基因座/花粉育性/高温胁迫分类
农业科技引用本文复制引用
盘毅,罗丽华,邓化冰,张桂莲,唐文邦,陈立云,肖应辉..水稻开花期高温胁迫下的花粉育性QTL定位 [J].中国水稻科学,2011,25(1):99-102,4.基金项目
国家自然科学基金资助项目(30971745, 30900874) （30971745, 30900874）
湖南省自然科学基金资助项目(08JJ1003) （08JJ1003）
教育部博士点基金资助项目(20070537006) （20070537006）
湖南省教育厅资助项目 (06B042). （06B042）
