中国水稻科学2011，Vol.25Issue(1)：52-58,7.DOI:10.3969/j.issn.1001-7216.2011.01.008
普通野生稻苗期耐冷性QTL的鉴定与分子定位
Identification and Mapping of QTLs for Cold Tolerance at the Seedling Stage in Common Wild Rice(Oryza rufipogon)
摘要
Abstract
Two core accessions of common wild rice (DP15 and DP30) as donor parents and the cult ivar 9311 as recipient parent were used to construct chromosome segment substitution lines for identification of QTLs for cold tolerance at the seedling stage and QTL effect analysis. A QTL mapping population from the most cold-tolerant substitution line was constructed to map major-effect QTLs with SSR markers. The two donor lines, DP15 and DP30, showed obvious difference in the number, loca-tion and effect of QTLs for cold tolerance. In the 230 BC4 F2 substitution lines in which substituted chromosome segments cov-ered the whole genome of the two donors, a total of 19 cold tolerance QTLs were detected, which distributed throughout all the 12 chromosomes, and clustered on chromosome 3 and chromosome 8. These QTLs were separated each other in different chromosome substitution lines. The substitution line with the smallest effect QTL for cold tolerance and the line with the lar-gest effect QTL showed 8％ and 74 ％ of survival rate after cold treatment, respectively. A main-effect QTL, qSCT-3-1 , was mapped between SSR markers RM15031 and RM3400, near the centromere on the long arm of chromosome 3 with a distance of 1.8 cM to the closest linkage markers, RM15040 and RM1164.关键词
耐冷性/数量性状基因座/普通野生稻/染色体片段代换系/基因定位分类
农业科技引用本文复制引用
郑加兴,黄金艳,陈淼,蒙姣荣,李容柏,陈保善,马增凤,宋建东,刘驰,李永桃,黄大辉,韦绍丽,张月雄,密克..普通野生稻苗期耐冷性QTL的鉴定与分子定位 [J].中国水稻科学,2011,25(1):52-58,7.基金项目
国家自然科学基金资助项目(30860142) （30860142）
国家转基因生物新品种培育科技重大专项资助项目(2009ZX08009-070B) （2009ZX08009-070B）
广西亚热带生物资源保护利用重点实验室开放课题资助项目(SB0604) （SB0604）
广西科学研究与技术开发计划资助项目(桂科能0630006-5F, 桂科能0815011-6-1). （桂科能0630006-5F, 桂科能0815011-6-1）
