临床神经病学杂志2011，Vol.24Issue(1)：30-33,4.
核因子-κB活性抑制剂对癫(癎)大鼠的脑保护作用
Brain protective effect of nuclear factor-κB activative inhibitor in epileptic rats
摘要
Abstract
Objective To investigate the brain protective effect of nuclear factor-κB (NF-κB) activity inhibitor pyrrolidine dithio-carbamate ( PDTC ) in epileptic (EP) rats.Methods Thirty-six male SD rats were randomly divided into EP group ( n = 14 ), PDTC intervention group ( PDTC group, n = 14 ) and sham operation group ( n = 8 ).The EP models were made by kainic (KA) injected into rat hippocampus.The rats in PDTC group were given PDTC 150 mg/kg by intraperitoneal injection 30 min prior to KA.The latency period of EP seizure and the time from the first to 6th ≥ Ⅳ grades seizure ( the seizure severity ) were observed in each group.HE staining and immunohistochemistry staining were used to detect the survival neurons and NF-κB expression in hippocampus CA3 region in each group.Results The EP seizure latency in PDTC group [( 89.6 ± 39.3 ) min]was longer than EP group [(67.5 ± 22.9) min],but there was no significant difference between the two groups.The time from the first to 6th ≥ Ⅳ grades seizure in PDTC group [(29.2 ± 20.4) min]was remarkably prolonged than EP group [( 12.1 ±4.0) min]( P ＜ 0.05 ).Compared with EP group, the guantity of survival neurous was significantly enhanced ( P ＜O.05), NF-κB expression was significantly down-regulated (P ＜ O.01 ) and in hippocampus CA3 area in PDTC group.There was a negative correlation between NF-κB expression and the guantity of survival neurous(r = -0.562,P =0.001 ).Conclusions NF-κB activity inhibitior can reduce the seizure severity and neuronal degeneration,which has the brain protective effect.It is suggested that brain damage associated with seizures may be related with NF-κB activation.关键词
癫(癎)/核因子-κB/吡咯烷二硫代氨基甲酸盐/脑保护作用Key words
epilepsy/ nuclear factor-κB/ pyrrolidine dithio-carbamate/ brain protective effect分类
医药卫生引用本文复制引用
余年,狄晴,胡勇,蒋颖,晏玉奎..核因子-κB活性抑制剂对癫(癎)大鼠的脑保护作用[J].临床神经病学杂志,2011,24(1):30-33,4.基金项目
南京市医学科技发展重大项目(2007-0211) （2007-0211）
