南京医科大学学报（自然科学版）2011，Vol.31Issue(3)：328-334,7.
神经导向因子Netrin-1基因转染大鼠骨髓间充质干细胞的体外实验
A study on transfecting neuronal guidance factor Netrin-1 to rat bone mesenchymal stem cells in vitro
摘要
Abstract
Objective:To study the effect of transfecting the neuronal guidance factor Netrin-1 and enhanced green fluorescent protein gene(EGFP) to rat bone mesenchymal stem cells(BMSCs) by adenovirus on the cytokine secretion, differentiation and tube formation.Methods:BMSCs were separated and purified in vitro.Surface receptors of adenovirus infected BMSCs and cytokines were detected by real-time PCR or RT-PCR.Tube formation of HUVEC was observed by coculture with BMSCs.BMSCs migration was observed by Transwell experiment.Results:Adenovirus infected BMSCs expressed some kinds of Netrin-1 receptors and secreted vascular endothelial growth factor(VEGF),basic fibroblast growth factor(bFGF),brain derived neurophic factor(BDNF) and nerve growth factor (NGF).BMSCs cultured with Netrin-1 and VEGF markedly increased the tube formation of HUVEC and BMSCs migration,compared with Netrin-1 or VEGF alone(P＜ 0.05).Conclusion:BMSCs successfully infected with Ad5-Netrin-1-CMV-EGFP can express EGFP and Netrin-1.Netrin-1 has the ability to accelerate tube formation and BMSCs migration and VEGF can enhance the effect when used together.关键词
Netrin-1/骨髓间充质干细胞/小管形成/Transwell迁移Key words
Netrin-1/ BMSCs/ tube formation/ Transwell migration分类
医药卫生引用本文复制引用
柯先金,李倩,孙波,张云云,姜婧,曹菁菁,黄素素,丁新生..神经导向因子Netrin-1基因转染大鼠骨髓间充质干细胞的体外实验[J].南京医科大学学报（自然科学版）,2011,31(3):328-334,7.基金项目
国家自然科学基金(30971022) （30971022）
江苏省自然科学基金(BK2008471) （BK2008471）
南京市医学科技发展项目(ZKX08014)资助 （ZKX08014）
