南京医科大学学报（自然科学版）2011，Vol.31Issue(3)：404-409,6.
2型糖尿病患者皮下、大网膜脂肪组织脂联素mRNA表达变化及意义
Expression and significance of adiponectin mRNA in subcutaneous and omental adipose tissue of type 2 diabetic patients
摘要
Abstract
Objective: To observe the adiponectin mRNA expression in subcutaneous and omental adipose tissue of type 2 diabetic patients and their relation with serum adiponectin, body mass index(BMI), waist circumference (WC),waist hip ratio (WHR), blood pressure, fasting plasma glucose (FPG), HbA 1 c, blood lipids and uric acid (UA). Methods:Twenty-three T2DM patients with obesity,23 T2DM patients, 33 obesity patients and 23 controls were selected. The adiponectin mRNA expression level in adipose tissue was assayed by RT-PCR. The level of serum adiponectin was measured with ELISA kit. FBG, triglyceride (TG), total cholesterol(TC),high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), HbAlc, UA of all subjects were determined and their blood pressure, height, weight, waist circumference, hip circumference were measured to calculate BMI,WHR.Results: ①The SBP, DBP, FPG, HbA1c, TG, LDL-C, UA were significantly higher, and serum adiponectin, HDL-C and adiponectin mRNA expression level in both subcutaneous and omental adipose tissue were significantly lower in diabetic obesity group than in obesity group (P ＜ 0.05), the adiponectin mRNA expression level in omental were significantly lower than in subcutaneous adipose tissue in both groups (P ＜ 0.01). ②The FPG, HbAlc, TG, UA were higher, serum adiponectin, HDL-C and adiponectin mRNA levels expressed in both subcutaneous and omental adipose tissue were lower in diabetic group without obesity than in control group without obesity (P ＜ 0.05), the adiponectin mRNA expression level in omental was lower than in subcutaneous adipose tissue in both groups (P ＜ 0.01). ③The BMI,WHR,SBP,DBP,FPG,HbA1C,TG,UA were higher, serum adiponectin and adiponectin mRNA level expressed in both subcutaneous and omental adipose tissue were lower in diabetic obese group than in diabetic group without obesity(P ＜ 0.05). ④The adiponectin mRNA expression level in omental adipose tissue of diabetic obesity group was negatively correlated to BMI, WHR, FPG (P ＜ 0.05),and was positively correlated to serum adiponectin (P ＜ 0.01 ). Conclusion:T2DM patients with obesity show down-regulated expression of adiponectin mRNA in omental adipose tissue and lower serum adiponectin than control obese group, BMI, WC, WHR, FPG are correlated with adiponectin mRNA expression and insulin resistance.关键词
脂联素/2型糖尿病/体质指数/胰岛素抵抗Key words
adiponectin/ type 2 diabetes mellitus/ body mass index/ insulin resistance分类
医药卫生引用本文复制引用
李倩倩,蒋秀琴,李慧敏,缪珩..2型糖尿病患者皮下、大网膜脂肪组织脂联素mRNA表达变化及意义[J].南京医科大学学报（自然科学版）,2011,31(3):404-409,6.基金项目
南京医科大学重点课题基金资助(07NMUZ014) （07NMUZ014）
