南京医科大学学报（自然科学版）2011，Vol.31Issue(3)：380-383,4.
枯草溶菌素转换酶9在大鼠心肌缺血再灌注损伤中的表达变化
Expression of proproteinconvertase subtilisin/kexin type 9 in myocardial ischemia-reperfusion injury in rats
摘要
Abstract
Objective: To investigate the expression of proproteinconvertase subtilisin/kexin type 9(PCSK9) in the rats myocardium impaired by ischemia-reperfusion. Methods:The left descending coronary artery(LAD) of rats was occluded for 45 min, followed by repeffusion of 6,12, or 24 h, respectively. The levels of creatine kinase(CK), creatine kinase MB(CK-MB) and lactate dehydrogenase(LDH) in serum were measured by automatic biochemical analyzer. The pathological changes in myocardium of rat were examined by HE staining. The expression of PCSK9 in myocardium was detected by RT-PCR and Western blot. Results:The levels of CK,CK-MB and LDH were significantly increased after 6 h and 12 h of reperfusion, and restored to normal level after 24 h of repeffusion. The reperfusion-induced myocardial damage increased in a time-dependent manner. The expression of PCSK9 mRNA and protein were significantly and time-depentently increased by ischemia-repeffusion injury. Conclusion:PCSK9 may be invovled in the myocardial ischemia-repeffusion injury.关键词
心肌缺血再灌注损伤/枯草溶菌素转换酶9/表达Key words
myocardial ischemia-reperfusion injury/ PCSK9/ expression分类
医药卫生引用本文复制引用
唐志晗,曾海燕,曾高峰,屈顺林,唐海林,刘录山,姜志胜..枯草溶菌素转换酶9在大鼠心肌缺血再灌注损伤中的表达变化[J].南京医科大学学报（自然科学版）,2011,31(3):380-383,4.基金项目
湖南省应用基础研究计划重点项目(2008FJ2006) （2008FJ2006）
湖南省科技厅计划项目(2009TP4057-2) （2009TP4057-2）
湖南省教育厅重点科研项目(10A105)及湖南省高校科技创新团队支持计划资助 （10A105）
