中国药业2011，Vol.20Issue(2)：17-19,3.
天麻药材高效液相色谱指纹图谱研究
HPLC Fingerprint of Gastrodia elata Blume
黄小玲 1杨春燕 1袁慧文2
作者信息
- 1. 广东省深圳市慢性病防治中心,广东,深圳,518020
- 2. 广东省深圳市药品检验所,广东,深圳,518029
- 折叠
摘要
Abstract
Objective To study the HPLC fingerprint of medicinal material Gastrodia elata Blume to provide the reliable method for its quality control. Methods The analysis was performed on Ultimate XB- C18 column (250 mm ×4. 6 mm, 5 μm) with the mobile phase of methanal- 1％ glacial acetic acid aqueous solution in the gradient elution. The column temperature was 30 ℃ and the flow rate was 1. 0 mL/min. The UV detector was set at 270 nm. Results The HPLC fingerprint of Gastrodia elata Blume was preliminarily established and 16 common peaks were displayed in the fingerprint. The average sample recovery rate of gastrodine was 102. 66％, RSD ＜ 2. 5％ ( n =6). Conclusion The HPLC fingerprint may provide a new model for controlling the quality of medicinal material Gastrodia elata Blume and also may provide an accurate and rapid tool for identifying unknown sample of Gastrodia elata Blume.关键词
天麻/高效液相色谱法/指纹图谱/质量控制/天麻素分类
医药卫生引用本文复制引用
黄小玲,杨春燕,袁慧文..天麻药材高效液相色谱指纹图谱研究[J].中国药业,2011,20(2):17-19,3.
