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加拿大一枝黄花不同部位酚类化合物的含量测定

王建方 陈佳铭 吴德峰 方波 卢艳花

中国中医药信息杂志2011,Vol.18Issue(2):59-61,3.
中国中医药信息杂志2011,Vol.18Issue(2):59-61,3.DOI:10.3969/j.issn.1005-5304.2011.02.024

加拿大一枝黄花不同部位酚类化合物的含量测定

Determination of Phenolic Compounds from Different Fractions of Solid-go Canadensis

王建方 1陈佳铭 2吴德峰 2方波 1卢艳花1

作者信息

  • 1. 华东理工大学生物反应器工程国家重点实验室,上海,200237
  • 2. 上海朝翔生物技术有限公司,上海,201611
  • 折叠

摘要

Abstract

Objective To develop the method for determination of phenolic compounds from different fractions of Solidago Canadensis. Methods Contents of phenolic compounds were determined by HPLC. The absorption was set at 360 nm and analysis was carried out at 30 ℃ on an Agilent ZORBAX Eclipse SB-C18 column (4.6 mm × 250 mm, 5 μm). The mobile phase consisted of acetonitrile and water, using a gradient program and the flow rate was kept at 1.0 mL/min. Results Contents of phenolic compounds in flower were higher than those in other fractions. Conclusion The developed HPLC assays were effective, accurate and reliable, and could be readily utilized for determination of phenolic compounds from different fractions of Solidago Canadensis.

关键词

加拿大一枝黄花/不同部位/酚类化合物/高效液相色谱法/含量测定

分类

医药卫生

引用本文复制引用

王建方,陈佳铭,吴德峰,方波,卢艳花..加拿大一枝黄花不同部位酚类化合物的含量测定[J].中国中医药信息杂志,2011,18(2):59-61,3.

基金项目

上海市松江区博士后创新实践基地和上海市科研资助计划(08R214214) (08R214214)

上海市大学生创新性实验计划 ()

中国中医药信息杂志

OACSCDCSTPCD

1005-5304

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