兰州大学学报（医学版）2011，Vol.37Issue(1)：6-10,5.
重组人脂联素融合蛋白在大肠埃希菌BL21中的表达
Expression, purification characterization of recombination human adiponectin in Escherichia coli BL21
杨霄鹏 1吕同德 1刘斌 2王晓辉 1唐瑜 1贾庆华1
作者信息
- 1. 兰州军区兰州总医院医学实验中心,甘肃,兰州,730050
- 2. 兰州军区兰州总医院病理科,甘肃,兰州,730050
- 折叠
摘要
Abstract
Objective To construct the recombinant adiponectin plasmid, induce the pET-32a(+) /AP expression in Escherichia coli BL21, and purify as well as identify its products. Methods The recombinant pET-32a/AP and pET-32a(+) were transformed into Escherichia coli BL21,made to induce and express. The expression system was built and the high expressed clone was selected. The best induction condition was confirmed by using different induction time and different isopropyl thiogalactoside induction concentration. The recombinant adiponectin was proceeded much more induction and expression, and the albumen was taken affinity chromatograph through the Ni2+ chromatographic column, and the purified albu en was concentrated and finally induced albumen was identified by westen-blotting. Results The westen-blotting identified that the marker 43 kD was the recombinant adiponectin fusion alumen. Conclusion The reco binant adiponectin fusion albumen is successfully induced and purified, which provides a good basis for further investigation of its physiological mechanism.关键词
脂联素/大肠埃希菌BL21/表达/诱导/纯化分类
生物科学引用本文复制引用
杨霄鹏,吕同德,刘斌,王晓辉,唐瑜,贾庆华..重组人脂联素融合蛋白在大肠埃希菌BL21中的表达[J].兰州大学学报（医学版）,2011,37(1):6-10,5.
