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探讨丙氨酸氨基转移酶IFCC法检测试剂中加入5'-磷酸吡哆醛对结果的影响

张莹 周铁成 童开 李晓柯

现代检验医学杂志2011,Vol.26Issue(1):85-86,89,3.
现代检验医学杂志2011,Vol.26Issue(1):85-86,89,3.DOI:10.3969/j.issn.1671-7414.2011.01.031

探讨丙氨酸氨基转移酶IFCC法检测试剂中加入5'-磷酸吡哆醛对结果的影响

Study on the Results of Alanine Aminotransferase Assay Reagent with Pyridoxal-5'-Phosphoric Acid

张莹 1周铁成 1童开 1李晓柯1

作者信息

  • 1. 第四军医大学西京医院全军临床检验中心,西安,710032
  • 折叠

摘要

Abstract

Objective To study alanine aminotransferase detect reagent with pyridoxal-5'-phosphoric acid. Methods Used IFCC primary reference procedures for the detect of alanine aminotransferase. Measurement of catalytic activity concentra tions of according to method by alanine aminotransferase detect reagent with or without pyridoxal-5'-phosphoric acid. Results As compared 22 tests the concentrations detected by alanine aminotransferase detect reagent with or without pyridoxal-5 ' -phosphoric acid ,the regression equation was Y= 0. 854X+1. 305 9 ,R2 = 0. 975 8. But there were some difference between the method by alanine aminotransferase detect reagent with pyridoxal-5'-phosphoric acid and without pyridoxal5 ' -phosphoric acid (P<O. 05 ). Conclusion The relativity between alanine aminotransferase detect reagent with or without pyridoxal-5'-phosphoric acid was well and the concentration detected by alanine aminotransferase reagent with pyridoxal5'-phosphoric acid was higher than the reagent without pyridoxal-5'-phosphoric acid about 20%.

关键词

丙氨酸氨基转移酶/5'-磷酸吡哆醛/相关性

分类

医药卫生

引用本文复制引用

张莹,周铁成,童开,李晓柯..探讨丙氨酸氨基转移酶IFCC法检测试剂中加入5'-磷酸吡哆醛对结果的影响[J].现代检验医学杂志,2011,26(1):85-86,89,3.

基金项目

国家科技支持计划资金资助项目(2007BA105809). (2007BA105809)

现代检验医学杂志

OACSTPCD

1671-7414

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