中国组织工程研究与临床康复2011，Vol.15Issue(3)：450-452,3.DOI:10.3969/j.issn.1673-8225.2011.03.017
纳米级二氧化锆增韧羟基磷灰石生物复合陶瓷对兔骨髓基质干细胞增殖、分化的影响
Effects of nano-zirconium dioxide-toughened hydroxyapatite bioceramics on proliferation and differentiation of rabbit bone marrow stromal cells
摘要
Abstract
BACKGROUND:Research group has performed some preliminary studies pertaining to zirconia toughened nano-hydroxyapatite (HA-2rO2) bioceramics, mainly in the biomechanical compatibility, chemical stability, and biocompatibility. Among them, in vitro cell culture experiments are controllable and repeatable, can well reflect the material's biocompatibility.OBJECTIVE:To study the influence to the growth and differentiation of rabbit bone marrow stromal stem cells by HA-2rO2 or HA matenals.METHODS:The rabbit bone marrow stromal stem cells were cultured in beccos modified eagle's medium (DMEM) containing 20％fetal bovine serum, the subsequent cell passage was cultured in conditioned medium containing dexamethasone, beta-sodium glycerophosphate and ascorbic acid. The third passage osteoblasts were cultured in cell culture plate with materials at 1.0×108/L.The proliferation of all the cultured cells were observed at 1-10 days under inverted phase contrast microscope and the curve of cell-growth was plotted. The activity of alkaline phosphatase was detected. At 6 days of the culture, cells and composite materials were fixed with paraformaldehyde and observed under scanning electron microscope.RESULTS AND CONCLUSION:MTT assay showed the growth curve of cells cultured in two materials had no significant difference. The combined cultu re of rabbit bone marrow stromal cells can maintain normal fu nction of secreting alkaline phosphatase. Electron microscopy photographs also confirmed the cell adhesion on two kinds of materials. HA-2rO2 and HA show no adverse effect on the growth and differentiation of osteoblasts, with excellent compatibility.关键词
羟基磷灰石/二氧化锆增韧羟基磷灰石/成骨细胞/生物相容性/生物材料分类
医药卫生引用本文复制引用
唐月军,王心玲,周中华,吕春堂..纳米级二氧化锆增韧羟基磷灰石生物复合陶瓷对兔骨髓基质干细胞增殖、分化的影响[J].中国组织工程研究与临床康复,2011,15(3):450-452,3.基金项目
江苏省卫生厅课题(Z200710). （Z200710）
