郑州大学学报（医学版）2011，Vol.46Issue(1)：48-50,3.
锌对染氟大鼠生精细胞Bcl-2和Bax蛋白表达的影响
Effects of zinc on expressions of Bcl-2 and Bax protein caused by fluoride in spermatogenic cells of male rats
摘要
Abstract
Aim: To study the effects of zinc on expressions of Bcl-2 and Bax protein caused by fluoride in spermato-genic cells of male rats. Methods: A total of 50 Wistar male rats were randomly divided into control group, fluorine treatment group, fluorine and low-dose zinc treatment group, and fluorine and middle-dose zinc treatment group, fluorine and high-dose zinc treatment group. The content of NaF in testis was measured by using fluorine selective electrode. Changes of testosterone and expressions of Bcl-2, Bax protein in spermatogenic cells were respectively observed using the methods of ra-dioimmunoassay, immunohistochemical study. In addition,the quality of spermatozoa was observed. Results:The testosterone levels in serum of the three dose add zinc groups were higher than those of fluorine group and control group ( F = 129. 179, P < 0.001). Bcl-2 expression in spermatogenic cells of each zinc treatment groups was higher than that of fluorine treatment group(F =69.918 ,P < 0.001). Bax expression in spermatogenic cells and the testis fluoride content of mid-dose zinc treatment group were lower than those of fluorine treatment group( F = 52. 142 and 453. 812,P <0.001). Conclusion: Zinc could antagonize the reproductive toxicity of fluoride in male rats by increase Bcl-2 protein expression and decrease Bax protein expression in spermatogenic cells. Middle dose of zinc has the beet effect, high dose of zinc has no effect, even may has synergism with sodium fluoride.关键词
氟化钠/锌/雄性生殖/Bcl-2/Bax/大鼠Key words
sodium fluoride/ zinc/ male reproduction/ Bcl-2/ Bax/ rat分类
医药卫生引用本文复制引用
冯亚静,高利华,段丽菊,程学敏,张慧珍,崔留欣..锌对染氟大鼠生精细胞Bcl-2和Bax蛋白表达的影响[J].郑州大学学报（医学版）,2011,46(1):48-50,3.基金项目
河南省卫生厅科技攻关基金资助项目 200903008 （）
