兰州大学学报（医学版）2011，Vol.37Issue(1)：44-49,6.
钠/碘转运蛋白和促甲状腺激素受体在甲状腺癌中的表达及相关性
Expression and correlation of sodium iodide symporter and thyroid stimulating hormone receptor in thyroid carcinoma
摘要
Abstract
Objective To investigate the expression of sodium/iodide symporter (NIS) and thyroid stimulating hormone receptor (TSHR) as well as their correlation in thyroid carcinoma.Methods mRNA levels, quantificd by real-time flurescent quanti-tive polymerase chain reaction, and protein evaluated by immunohistochemistry, were examined in surgical specimens of 18 normal thyroid tissue, 27 nodular goiter and 23 thyroid carcinoma samples.Results The NIS and TSHR mRNA levels of thyroid carcinoma was significantly lower than in normal thyroid tissue and nodular goiter (P ＜0.005).There was a positive correlation between NIS and TSHR in nodular goiter and normal thyroid samples (r =0.667 and 0.551, respectively, P ＜0.005), there was no such correlation in thyroid carcinomas.The immunohistochemistry results of thyroid cancer showed that the NIS protein mainly located in cytoplasm, and was markedly higher than normal thyroid tissue and nodular goiter (P ＜0.05).TSHR protein mainly located in cytoplasm and cytomembrane in thyroid carcinomas, and was significantly higher when compared with normal thyroid tissues (P ＜0.001), but had no difference with nodular goiter.Through combination analysis of NIS mRNA concentrations and NIS immunostaining scores, among the thyroid carcinomas, reduction of NIS mRNA expression but with increment of intracellular protein was markedly higher than in nodular goiter (x2 =4.428, P =0.035).Conclusion In thyroid carcinoma, NIS and TSHR mRNA levels were low, also there was no significant correlation.关键词
甲状腺癌/钠/碘转运蛋白/促甲状腺激素受体/荧光定量分析/免疫组织化学Key words
thyroid carcinoma/ sodium/iodide symporter/ thyrotropic hormone receptor/ quantitative fluorescence analysis/ immunohistochemistry分类
医药卫生引用本文复制引用
王志峰,刘勤江,廖世奇,杨荣..钠/碘转运蛋白和促甲状腺激素受体在甲状腺癌中的表达及相关性[J].兰州大学学报（医学版）,2011,37(1):44-49,6.基金项目
甘肃省科技支撑计划项目(0804NKCA087) （0804NKCA087）
