核农学报2011，Vol.25Issue(1)：131-136,142,7.
丙酯草醚胁迫下靶标基因在油菜子叶胚状体中的表达特征
EXPRESSION CHARACTERISTICS OF TARGET GENES UNDER HERBICIDE ZJ0273 STRESS IN COTYLEDONARY EMBRYOS OF Brassica napus L.
摘要
Abstract
The response of acetolactate synthase (ALS) and ketol-acid reductoisomerase (KARI) enzymes as well as ALS and KARI genes to different concentrations of the herbicide ZJ0273 at cotyledonary embryo stage of Brassica napus were investigated, and effect of this novel herbicide on physiological activity and gene expression differences was clarified. Oilseed rape variety Zheshuang 758, with good response to microspore culture, was subjected to various ZJ0273 treatments （0, 1, 10 mg/L) and the expressions of two genes (ALS and KARI) encoding ALS and KARI in leaves were detected by using real-time fluorescence quantitative PCR (FQ-PCR). The results showed that the effects of low ZJ0273 concentration (1 mg/L) on the expression of ALS and KARI genes were not significant, while down regulation was observed for ALS and KARI genes under high ZJ0273 concentration (10mg/L). These results indicated that ALS and KARI genes were involved in the regulation of differentiation of cotyledonary embryos under herbicide ZJ0273 stress, and ALS and KARI genes are the possible target genes for herbicide ZJ0273.关键词
油菜/子叶胚状体/丙酯草醚/乙酰乳酸合成酶/酮醇酸还原异构酶/基因表达/实时荧光定量/PCRKey words
Brassica napus L./ cotyledonary embryo/ ZJ0273/ ALS/ KARI/ gene expression/ real-time fluorescence quantitative PCR引用本文复制引用
万光龙,耿鑫鑫,吕晓,刘宏波,郭翔,罗茜,汪海燕,周伟军..丙酯草醚胁迫下靶标基因在油菜子叶胚状体中的表达特征[J].核农学报,2011,25(1):131-136,142,7.基金项目
国家科技支撑计划(2010BAD01B04),国家自然科学基金(30871652,31000678,31071698),国家油菜产业体系专项(nycytx-005),国家大学生创新性实验计划. （2010BAD01B04）
