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紫外分光光度法测定转基因烟草叶片中白藜芦醇的含量

毕琮 李臻 刘倩 刘炜

山东农业科学Issue(1):93-96,4.
山东农业科学Issue(1):93-96,4.

紫外分光光度法测定转基因烟草叶片中白藜芦醇的含量

Determination of Resveratrol in Tran sgenic Tobacco Leaves by UV Spectrophotometry

毕琮 1李臻 1刘倩 2刘炜1

作者信息

  • 1. 山东省农业科学院高新技术研究中心/山东省作物遗传改良与生物技术重点实验室,山东,济南,250100
  • 2. 农业部黄淮海作物遗传改良与生物技术重点开放实验室,山东,济南,250100
  • 折叠

摘要

Abstract

The resveratrol content in transgenic tobacco leaves was determined by UV spectrophotometry.The results showed that the resveratrol concentration in transgenic tobacco leaves was about 6.83 μg/ml,which was slightly higher than that in wild type material (control) of 5.11 μg/ml. Under the UV stress, the resveratrol contents in transgenic material and control were up to 10.98 and 9.25 μg/ml respectively. The resveratrol synthetic enzyme gene could not only increase the resveratrol content, but also improve the tolerance of tobacco to UV stress.

关键词

紫外分光光度法/烟草/花生白藜芦醇合成酶/白藜芦醇/紫外胁迫

Key words

UV spectrophotometry/ Tobacco/ Peanut resveratrol synthetic enzyme/ Resveratrol/ UV stress

分类

化学化工

引用本文复制引用

毕琮,李臻,刘倩,刘炜..紫外分光光度法测定转基因烟草叶片中白藜芦醇的含量[J].山东农业科学,2011,(1):93-96,4.

基金项目

山东省农业科学院博士科研启动基金项目(NO.2007YBS008)资助. (NO.2007YBS008)

山东农业科学

OACSTPCD

1001-4942

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