动物营养学报2011，Vol.23Issue(3)：480-485,6.DOI:10.3969/j.issn.1006-267x.2011.03.019
抗草甘膦转基因大豆及其加工产品的环介导等温扩增检测技术
A Loop-mediated Isothermal Amplification to Detect Genetically Modified Soybean Resistant to Glyphosate and its Processed Products
摘要
Abstract
The study was conducted to set a detection method for exogenous gene of genetically modified ( GM) soybean resistant to glyphosate and its processed products by loop-mediated isothermal amplification ( LAMP) method. Four specific primers were designed according to the 6 distinct sequences on the target region of CP4EPSPS gene. Samples of soybean, soybean meal and feedstuff containing soybean were detected by LAMP method. The results indicated that the CP4-EPSPS gene was successfully detected by LAMP method and the sensitivity was 100 to 1000 fold higher than PCR. The LAMP method is easy to operate and can be used for the rapid identification of GM soybean and its processed products. [ Chinese Journal of Animal Nutrition , 2011 , 23(3) :480-485]关键词
环介导等温扩增/转基因大豆/加工产品分类
农业科技引用本文复制引用
田芳,王秀敏,滕达,杨雅麟,管庆丰,敖长金,王建华..抗草甘膦转基因大豆及其加工产品的环介导等温扩增检测技术[J].动物营养学报,2011,23(3):480-485,6.基金项目
国家转基因生物新品种培育科技重大专项,转基因生物饲料检测与监测技术No.2009ZX08012～012B （）
