湖泊科学2011，Vol.23Issue(2)：271-276,6.
四个鲤鱼种群ITS-1序列的遗传变异分析
Sequence variation of Ribosomal DNA Intemal Transcribed Spacer 1 of four common carp populations
摘要
Abstract
The internal transcribed spacer 1 ( ITS-1 ) of four common carp populations were amplified and sequenced.The result showed that the average contents of GC were obviously higher than that of AT in the aligned sequences of 370bp.Total 34 variable sites were detected, and 14 haplotypes were recovered.The haplotype diversity and nucleotide diversity were 0.637 ± 0.055 and 0.00857 ±0.00200, respectively.The genetic distance between Heilongjiang carp and Jian carp displayed the highest, up to 0.10129, while that between Heilongjiang carp and Yellow River carp populations was the lowest to 0.02305.The fixation indices ( Fst ) of analysis of molecular variance (AMOVA) among populations was 0.05373, which showed no significant population stmcture.The phylogenetic tree of ITS-1 built with MEGA 4.1 showed that four common carp populations were clustered into two major clades based on genetic distance.Populations of Heilongjiang carp and Yellow River carp were clustered in northern clade, while Jian carp and Hebao red carp were clustered in southern clade.关键词
鲤鱼/内转录间隔区1(ITS-1)/种质资源/遗传变异Key words
Common carp/ internal transcribed spacer 1/ genetic resources/ genetic diversity引用本文复制引用
钟立强,张成锋,周凯,李冰,王建新,朱健..四个鲤鱼种群ITS-1序列的遗传变异分析[J].湖泊科学,2011,23(2):271-276,6.基金项目
中央级公益性科研院所基本科研业务费专项基金项目(2009JBFB14)、现代农业产业技术体系建设专项资金项目(NYCYTX-49)和农业部种质资源保护项目联合资助. （2009JBFB14）
