中国病理生理杂志2011，Vol.27Issue(2)：315-319,5.DOI:10.3969/j.issn.1000-4718.2011.02-020
心理应激对大鼠实验性牙周炎及牙周组织中低氧诱导因子1α表达的影响
Effect of psychological stress on development of periodontitis and expression of periodontal hypoxia-inducible factor 1α in rats
摘要
Abstract
AIM: To investigate the effect of psychological stress on the development of periodontitis and the expression of periodontal hypoxia - inducihle factor 1α( HIF - 1α ) in rats.METHODS: Forty - eight male Wistar rats ( SPF grade ) were randomly divided into 4 groups :( 1 ) normal control group, i.e.naive rats ;( 2 ) experimental periodontitis group : the periodontitis model was induced by wrapping 3 - 0 silk ligature inoculated with putative periodontopathic bacteria around the left maxillary second molar of the rats;( 3 ) stress group : the rats were treated with stress alone;( 4 ) periodontits with stress group, the periodontitis model was induced as ahove , and the rats were treated with stress.The rats were sacrificed at week 1 , 4, 6 and 8 after the ligature.The attachment losses( AL ) were measured by home - made probe.The histological changes of periodontal tissues stained with hematoxylin and eosin( HE ) were observed under microscope.The HIF - 1α expression level in the periodontal epithelium was determined by immunohistochemistry that was used to evaluate the severity of hypoxia hy measuring the average rate of HIF - 1α - positive cells.RESULTS : No significant difference of AL between stress group and normal control group was observed( P ＞ 0.05 ).The AL and the average rate of HIF - 1α positive cells in periodontitis with stress group were significantly higher than those in experimental periodontitis group at time points of week 4,6 and 8 after ligature( hoth P ＜ 0.01 ).CONCLUSION : Psychological stress is one of the periodontitis inducing factors in the animal model.Psychological stress may aggravate periodontitis hy decreasing tissue oxygenation in rats.关键词
牙周炎/动物模型/心理应激/低氧诱导因子1α分类
医药卫生引用本文复制引用
张增方,吕芳丽,陈莹,杨晓燕,黄绮凌,李东健,黄世光..心理应激对大鼠实验性牙周炎及牙周组织中低氧诱导因子1α表达的影响[J].中国病理生理杂志,2011,27(2):315-319,5.基金项目
国家自然科学基金资助项目(No.81071387) （No.81071387）
广东省自然科学基金资助项目(No.10151008901000006) （No.10151008901000006）
