中国组织工程研究与临床康复2011，Vol.15Issue(6)：1057-1060,4.DOI:10.3969/j.issn.1673-8225.2011.06.023
脂肪基质细胞构建组织工程骨修复下颌骨缺损的实验研究
Experimental research on adipose-derived stromal cells in tissue engineering bone for the repair of canine mandibular bone defects
李晓宇 1姚金凤 2刘政华 1蔡剑波1
作者信息
- 1. 深圳市宝安区人民医院口腔颌面外科,广东省深圳市,518101
- 2. 深圳市第二人民医院口腔科,广东省深圳市,518035
- 折叠
摘要
Abstract
BACKGROUND:Previous studies demonstrated that only adipose-derived stromal cells (ADSCs) after osteogenic induction can be served as seeding cells for bone tissue engineering. However, the process of osteogenic induction is complicated, making the procedure expensive and elongating the culture time in vitro.OBJECTIVE: To explore the feasibility of repairing the canine mandibular bone defects with tissue engineering method based on non-osteogenically induced ADSCs as seeding cells.METHODS: The subcutaneous fat from the back of dogs aged 12 months were obtained, mononuclearcell was obtained by collagenase digestion method. The cultured 3rd passage cells and biphasic calcium phosphate (BCP) ceramics formed scaffold composite. 20 mm in length and 10 mm in height of box-like bone defects were prepared in both sides of canine mandibular; the teeth were extracted in defect region. The cell-scaffold composite and BCP ceramics scaffold were implanted in canine mandibular bone defects. The region without intervention served as control. The repair of bone defects was detected by histology,at 4 weeks and 8 weeks implantation.RESULTS AND CONCLUSION : After 4 weeks scaffold im plantation, part of scaffold materials was degraded; new bone was formed in defect region. The bone formation in BCP group was significantly less than that in cell-scaffold composite group, and formed a small amount of new bones and part of the new vessels. At 8 weeks scaffold implantation, more new bones were formed between BCP group and cell-scaffold composite group, which were widely distributed in the bone defect region. However,the new bones in BCP group were still significantly less than that in cell-scaffold composite group; there were significant differences (P ＜ 0.01). It is indicated that ADSCs combine with BCP can promote bone formation in vivo. ADSCs-scaffold construct can promote bone formation in vivo. Non-osteogenically induced ADSCs can be used as seed cells to repair the mandibular bone defects by tissue engineering technology.关键词
脂肪基质细胞/组织工程/双相磷酸钙陶瓷/颌骨缺损/支架分类
医药卫生引用本文复制引用
李晓宇,姚金凤,刘政华,蔡剑波..脂肪基质细胞构建组织工程骨修复下颌骨缺损的实验研究[J].中国组织工程研究与临床康复,2011,15(6):1057-1060,4.
