中国当代儿科杂志2011，Vol.13Issue(1)：53-56,4.
二烯丙基二硫诱导人白血病K562细胞凋亡及其对Fas、FasL及caspase-8表达的影响
Effects of diallyl disulfide Oil apoptosis of human leukemia K562 cells and expression of Fas,FasL and caspase-8
摘要
Abstract
Objective To study the effects of diallyl disulfide (DADS) on apoptosis of human leukemia K562 cells and possible mechanisms. Methods The morphologic changes of leukemia K562 cells after DADS treatment were observed by Hoechst 33258 staining. Cell apoptosis rates after different concentrations and different durations of DADS treatment were determined by flow cytometry. Fas, FasL and caspase-8 mRNA expression was estimated by reverse transcriptionpolymerase chain reaction (RT-PCR) 48 hrs after DADS treatment. Results The characteristics of apoptosis in K562 cells induced by DADS were observed. After 24 hrs of DADS treatment, the apoptosis rate of K562 cells increased from ( 11.60 ± 0.83 )％ at the concentration of 10 mg/L to (37.94 ± 0.87 )％ at the concentration of 40 mg/L. The apoptosis rate of K562 cells increased after 40 mg/L DADS with the increasing time from ( 37.94 ± 0. 87 ) ％ ( 24 hrs ) to ( 47.02 ±0.66)％ (72 hrs ). Expression of Fas and caspase-8 mRNA increased, while FasL mRNA expression decreased significantly 48 hra after DADS treatment compared with the control group ( P ＜ 0.05 ). Conclusions DADS can induce apoptosis of human leukemia K562 cells in a time- and concentration-dependent manner, possibly through increasing Fas and caspase-8 expression and decreasing FasL expression.关键词
二烯丙基二硫/凋亡/K562细胞分类
医药卫生引用本文复制引用
肖正香,殷小成,谭艳芳,彭艳辉..二烯丙基二硫诱导人白血病K562细胞凋亡及其对Fas、FasL及caspase-8表达的影响[J].中国当代儿科杂志,2011,13(1):53-56,4.基金项目
湖南省高校科研经费项目(09C840). （09C840）
