农药学学报2011，Vol.13Issue(1)：53-58,6.DOI:10.3969/j.issn.1008-7303.2011.01.09
三唑醇对映体的反相高效液相色谱拆分热力学研究
Thermodynamic property of the enantiomeric separation for triadimenol by reverse-phase high-performance liquid chromatography
摘要
Abstract
Enantiomeric separation of triadimenol was performed by reverse-phase high-performance liquid chromatography ( HPLC ) on the coating-type cellulose tris ( 3,5-dimethylphenylcarbamate )chiral stationary phase (CDMPC-CSP). The effects of different mobile phase compositions and temperature of column on the retention and resolution in the chiral separation of triadimenol were investigated. Moreover, the enantiomeric separation mechanism of triadimenol enantiomer on the CDMPC-CSP was analyzed by thermodynamical parameters. The results showed that the ideal resolution of triadimenol on CDMPC-CSP could be achieved with the mobile phase of methanol-water (70:30,V/V) at a flow rate of 0.8 mL/min and at 15 ℃, or could be achieved with the mobile phase of acetonitrile-water (30:70, V/V) at a flow rate of 1.0 mL/min and at 45 ℃.关键词
三唑醇/对映体拆分/反相高效液相色谱/热力学Key words
triadimenol/ enantiomeric separation/ reverse-phase high performance liquid chromatography/ thermodynamics分类
医药卫生引用本文复制引用
王新全,王雪松,王祥云,徐浩,章虎,李振..三唑醇对映体的反相高效液相色谱拆分热力学研究[J].农药学学报,2011,13(1):53-58,6.基金项目
国家自然科学基金资助项目(20807038) （20807038）
浙江省农科院科技创新能力提升项目(2007R19Y01E02). （2007R19Y01E02）
