中国人兽共患病学报2011，Vol.27Issue(2)：141-143,3.
犬源环孢子虫的ITS-1序列鉴定
Molecular identification of dog-derived Cyclospora sp. based on ITS-1 sequence
摘要
Abstract
To identify one Cyalospora sp. from dog feces, a pair of primers ITS-1+F/ITS-1+B were designed to amplify the ITS-1 + region of the Cyclospora-like species from dog by PCR amplification based on the published sequence of the rDNA gene of Cyclospora in GenBank. The amplicons were purified and cloned into pMD-19T vector followed by being transfected into JM109. The positive clones were cultured and identified by PCR amplification and the insert was successfully sequenced. The result revealed that the amplicon was 547bp in length, including 58bp of 18S rDNA, 333bp of ITS-1 and 156bp of 5, 8S rDNA. The dog associated Cyclospora-like organism belongs to Eimeriidae based on the high similarity of its partial 18S rDNA and 5.8S rDNA and other protozoans of Eimeriidae. The organism might be a new species based on its specific ITS-1 sequence.关键词
犬源环孢子虫/内转录间隔区1/PCR/分子鉴定分类
医药卫生引用本文复制引用
岳彩玲,李国清,程家林,高振永,朱海波,李结..犬源环孢子虫的ITS-1序列鉴定[J].中国人兽共患病学报,2011,27(2):141-143,3.基金项目
国家自然科学基金(No.30671577,30371082)资助 （No.30671577,30371082）
