中山大学学报（医学科学版）2011，Vol.32Issue(1)：109-115,7.
十二个X染色体STR基因座荧光标记复合扩增体系的法医学应用
Forensic Application of Twelve X Chromosome STRs Fluorescence-labeled Multiplex Amplification System
摘要
Abstract
[Objective]To evaluate the multiplex PCR system of 12 X Chromosome STR loci (X-STR) for forensic application in Guangdong Han population.[Methods]DNA samples from 272 unrelated individuals (144 males and 128 females) were extracted and amplified with Investigator Argus X-12 multiplex PCR system labeled by five kinds of fluorescence.PCR products were separated and genotyped with capillary electrophoresis.[Results]A total of 272 samples were genotyped explicitly.Among them, 153 alleles were observed totally.All the loci showed no difference in sex-related allele frequency.No markers departed from Hardy-Weinberg equilibrium in female samples after Bonferroni's correction.DXS10103 and DXS10101 were found to be in linkage disequilibrium.The polymorphism information content (PIC) was 0.421 9 ～ 0.919 1.The discrimination power (DP) was 0.518 7 ～ 0.989 2.The mean exclusion chance (MEP)was 0.287 7 ～ 0.919 1.The combined discrimination power was 0.999 999 996 in males (CDPM) and 0.999 999 999 in females (CDPF), and the combined mean exclusion chance was 0.999 998 336 in duo cases (CMECduo),and 0.999 999 987 in trio cases (CMECtrio).[Conclusion]The 12 X-STR loci are powerful and reliable for forensic personal identification, paternity testing, especially for the testing of special paternity cases.关键词
X 染色体/短串联重复/复合扩增/法医学分类
生物科学引用本文复制引用
任峥,曾祥培,陈文静,吴晓洁,童大跃,李建金,孙宏钰..十二个X染色体STR基因座荧光标记复合扩增体系的法医学应用[J].中山大学学报（医学科学版）,2011,32(1):109-115,7.基金项目
教育部留学回国人员科研启动基金项目(2008-890号) （2008-890号）
上海市法医学重点实验室开放课题(KF0902) （KF0902）
清远市科技计划项目(2010A002) （2010A002）
