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南沙参中多糖的含量测定

梁莉 王婷 乔华 常威 李丹 张颍锴

中国药房2011,Vol.22Issue(11):1001-1003,3.
中国药房2011,Vol.22Issue(11):1001-1003,3.

南沙参中多糖的含量测定

Content Determination of Polysaccharide in Radix Adenophorae

梁莉 1王婷 1乔华 1常威 1李丹 1张颍锴2

作者信息

  • 1. 兰州大学第一医院,兰州市,730000
  • 2. 兰州大学药学院,兰州市,730000
  • 折叠

摘要

Abstract

OBJECTIVE: To establish the method for the content determination of polysaccharides in Radix Adenophorae.METHODS: The polysaccharide was isolated and extracted from Radix Adenophorae by water extraction and alcohol precipitation.The standard curve of polysaccharide from Radix Adenophorae was prepared with glucose as control. The content of polysaccharide was determined by anthrone-sulfuric acid method at detection wavelength of 625 nm. RESULTS: The content of polysaccharide from Radix Adenophorae was 38.5% with average recoveries of 101.4%. RSD was 2.83% (n=6). CONCLUSION: Anthrone-sulfuric acid method is simple, accurate and stable for the content determination of polysaccharide from Radix Adenophorae.

关键词

南沙参/多糖/水提醇沉/蒽酮-硫酸法/含量测定

分类

医药卫生

引用本文复制引用

梁莉,王婷,乔华,常威,李丹,张颍锴..南沙参中多糖的含量测定[J].中国药房,2011,22(11):1001-1003,3.

基金项目

2007年甘肃省中医药科研项目(甘卫中发[2007]123号) (甘卫中发[2007]123号)

中国药房

OACSCDCSTPCD

1001-0408

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