南方医科大学学报2011，Vol.31Issue(1)：138-140,3.
MRL/1pr狼疮鼠肾组织中氧化状态及γ-GCS的表达
Oxidation status and γ-glutamylcysteine synthetase expression in the kidney of MRL/lpr lupus mice
摘要
Abstract
Objective To investigate the role of glutathione (GSH) and γ-glutamylcysteine synthetase (γ-GCS) in lupus nephritis. Methods Spectrophotometry was used to measure the oxidative/anti-oxidative indices including malonyldialdehyed (MDA) and GSH in the kidney of MRL/lpr lupus mice. Quantitative PCR and Western blotting were used to detected the expression of γ-GCS. Results The level of GSH was lowered whereas the level of MDA increased significantly in the kidney tissue of MRL/lpr lupus mice as compared with that in normal control mice. The expression of y-GCS mRNA and protein was significantly decreased in MRL/lpr lupus mice (PO.05). Conclusion MRL/lpr lupus mice have abnormal oxidative stress in the kidney tissue, where the expression of γ-GCS decreased to lead to reduced GSH production, damaged antioxidative capacity, and eventually exacerbation of oxidative damage in the kidney.关键词
狼疮性肾炎/γ谷氨酰半胱氨酸合成酶/氧化应激Key words
lupus nephritis/ γ-glutamylcysteine synthetase/ oxidative stress分类
医药卫生引用本文复制引用
陈瑞林,陶怡,张北平,吕志芬..MRL/1pr狼疮鼠肾组织中氧化状态及γ-GCS的表达[J].南方医科大学学报,2011,31(1):138-140,3.基金项目
广东省自然科学基金(7003076) （7003076）
