四川大学学报（自然科学版）2011，Vol.48Issue(1)：206-212,7.DOI:10.3969/j.issn.0490-6756.2011.01.037
一种麦芽酸性磷酸酶的分离纯化及部分性质研究
Purification and some properties of wheat germ acid phosphatase
摘要
Abstract
A kind of acid phosphatase was purified from wheat germ.Some properties of the enzyme had been studied.The enzyme was obtained by ammunium sulfate fractionation, CM-Sepharose CL 4B ionexchange chromatography and DEAE-Sepharose CL 4B ion-exchange chromatography.The purification multiple was 33.06 and the specific activity was 88.27 U/mg.The optimum pH and optimum temperature were pH 5.4 and 50 ℃ respectively.Stability range of pH was just about 6.0.The enzyme was stable below 40 ℃.The Km of pNPP as substrate was 4.696 × 10-4mol/L at pH 5.4 and 50 ℃.The effects of some metal ions and organic compounds on the enzyme activity were studied.关键词
麦芽/酸性磷酸酶/分离纯化/动力学性质Key words
wheat germ/ acid phosphatase/ purification/ kinetic properties分类
生物科学引用本文复制引用
杨彩兰,陈巍,苟春宝,王勇,魏炜..一种麦芽酸性磷酸酶的分离纯化及部分性质研究[J].四川大学学报（自然科学版）,2011,48(1):206-212,7.基金项目
十一五国家科技支撑计划重点项目((2006BAF07B01) （(2006BAF07B01）
