癌变·畸变·突变2011，Vol.23Issue(1)：13-15,21,4.DOI:10.3969/j.issn.1004-616x.2011.01.004
维生素E和β-胡萝卜素对小鼠辐射损伤的联合作用
Combined effects of vitamin E and β-carotene on radiation injuries in mice
摘要
Abstract
OBJECTIVE: To study the antioxidation of vitamin E and/or β-carotene on mice induced by radiation. METHODS: Mice were divided randomly into 5 groups of normal control, positive, vitamin E, β-carotene,vitamin E plus β-carotene. All of the animals except the normal control were irradiated with 4.5 Gy of 60Coγ . After the radiation, the vitamin supplementation animals were given vitamin E [30 mg/(kg·d)] and/or β-carotene[20 mg/(kg· d)].Three days later, the animals were sacrificed. The retinol binding protein (RBP) in serum was analyzed with enzyme-linked immunosorbent assay (ELISA), and the levels of malondialdehyde (MDA) and reduced glutathione (GSH) in serum and liver were determined with fluorometry. RESULTS: 60Coγradiation decreased the level of RBP in serum. Supplementation of vitamin E and/or β-carotene showed certain protection. Vitamin E and/or β -carotene also inhibited the increase of MDA and the decrease of GSH in serum and liver induced by60Co γ radiation. Vitamin E alone showed the best protection.CONCLUSION: Both vitamin E and β-carotene showed protective effects on the oxidative injuries in mice induced by radiation. But the combined use of vitamin E and β-carotene showed antagonistic rather than synergistic effect.关键词
维生素E/β-胡萝卜素/抗氧化剂/辐射Key words
vitamin E/ β-carotene/ antioxidant/ radiation分类
医药卫生引用本文复制引用
郑怡麟,梁欣,海春旭,陈宏莉,刘瑞,秦绪军..维生素E和β-胡萝卜素对小鼠辐射损伤的联合作用[J].癌变·畸变·突变,2011,23(1):13-15,21,4.基金项目
本科生课外科研课题 （）
