水生生物学报2011，Vol.35Issue(1)：30-36,7.DOI:10.3724/SP.J.1035.2011.00030
厚壳贻贝低分子量足丝蛋白的初步鉴定
IDENTIFICATION OF MUSSEL FOOT PROTEINS WITH LOW MOLECULAR MASS FROM MYTILUS CORUSCUS PLAQUES
摘要
Abstract
Mytilidae owns a kind of special adhesive protein known as the mytilus foot protein (mfp) which provides secure adhesion in the presence of moisture and therefore become the main candidate for new biological adhesive developing at present.Possessing important economic value, Mytilus coruscus, with strong byssul and excellent adhesion,widely spreads all through the littoral extent of Zhejiang, China, while its foot proteins had rarely been studied.To understand the composition and the characterization of adhesive proteins from Mytilus coruscus several foot proteins were separated by C18 reverse-phase HPLC from the acetic acid-urea extracts of plaques and analyzed by matrix-assisted laser desorption ionization time of flight mass spectrometry to determine the mass ranging from3 to 6 kD followed with partially sequencing by Edman degradation.Three of these proteins, containing abundant amount of Dopa, were determined to be variants of a family known as mfp3 (mytilus foot protein 3) and the other three proteins were presumed to be novel types of foot proteins, because no results were matched after online BLAST search.Quartz crystal microbalance analysis assay showed that some of these proteins exhibited strong absorption abilities on gold surface, which directly correlated with their adhesion force.Notwithstanding its limitation, this study did build the foundation for further research on the molecular diversity of the foot proteins with low molecular mass from Mytilus coruscus and the related adhesion mechanism.关键词
厚壳贻贝/足丝蛋白/Mfp-3/DOPAKey words
Mytilus coruscus/Foot protein/Mfp-3/Dopa分类
生物科学引用本文复制引用
鲁涛,廖智,刘梅,叶茂,谭亮,王日昕..厚壳贻贝低分子量足丝蛋白的初步鉴定[J].水生生物学报,2011,35(1):30-36,7.基金项目
Research and Agricultural Project of Zhejiang Technology Department (2008C22026,2009C32016) （2008C22026,2009C32016）
Key Project of Zhejiang Education Department (20070430) （20070430）
Xin-Miao Project of Zhejiang Technology Department (2008R40G2110002) （2008R40G2110002）
