中国药理学通报2011，Vol.27Issue(3)：423-427,5.
小分子肽(KP)对小鼠成骨前体细胞MC3T3-E1增殖分化及NF-κB表达的影响
The effect of the small molecular weight peptide(KP) on the proliferation, differentiation of mouse MC3T3-E1 cells and the expression of NF-κB
摘要
Abstract
Aim To investigate the effect of the small molecular weight peptide( KP )on the proliferation and differentiation of mouse MC3T3-E1 cells and the expression of NF-KB p50, p65. Methods The cells growth , alkaline phosphatase ( ALP ) activity ,hydroxyproline content and mineralization of MC3T3El were measured by MTT, PNPP, visible spectrophotometry and alizarin red staining respectively. p65 , p50 protein expression in MC3T3-EI cells were detected by Western blot. Results KP enhanced cell proliferation after MC3T3-E1 cells was treated with KP ( 25. 0,50. 0.100 mg · L-1 )for 24 . 48 h and 72 h. Treated with KP at concentrations of 50. 0 and 100 mg . L-1 for 9,12, 15 d. ALP activity was significantly stimulated;and hydroxyproline contents was enhanced after being treamted 12 .24 d. KP had significant effect on mineralization at concentrations of 50. 0 mg . L-1 and 100 mg · L-1. After 50.0 mg · L-1 and 100 mg · L-1 KP treatment for 3 , 12 , 24 , 30 d , the expressions of p65 and p50 in MC3T3-E1 cells were gradually decreased.Conclusion KP induct MC3T3-EI cells proliferation and differentiation by inhibition of the NF-KB pathway.关键词
小分子肽/MC3T3-E1/增殖/分化/矿化/NF-κBp50/NF-κBp65分类
医药卫生引用本文复制引用
刘义,吴昆鹏,杨巧珠,李延平,崔燎..小分子肽(KP)对小鼠成骨前体细胞MC3T3-E1增殖分化及NF-κB表达的影响[J].中国药理学通报,2011,27(3):423-427,5.基金项目
广东省科技计划资助项目(No 2009B030801339,2010B 090400463) （No 2009B030801339,2010B 090400463）
广东省教育厅大学生创新实验项目(No KY1007) （No KY1007）
