重庆医学2011，Vol.40Issue(7)：646-649,后插3,5.DOI:10.3969/j.issn.1671-8348.2011.07.009
Pak1基因在乳腺癌组织中的表达及临床意义
Expression of Pak1 gene in breast carcinoma and its clinical significance
李秋云 1施俊义 1盛湲1
作者信息
- 1. 第二军医大学附属长海医院乳腺外科,上海,200433
- 折叠
摘要
Abstract
Objective To invcstigate mRNA and protein expression of Pak1 gene in normal breast tissue, benign mammary lesions and breast carcinoma,to explore whether the expression of Paklgene in breast carcinoma is related to lymph node metastases, the level of ER,PR and CerbB-2 or not. Methods The mRNA expression of Pak1 gene was detected in 35 cases of breast carcino ma,20 cases of benign mammary lesions and 15 cases of normal breast tissue by fluorescent realtime RT-PCR;35 cases of paraffinembedded specimens of breast carcinoma and 20 cases of benign mammary lesions were studied by immunohistochemical assay. Sta tistical analysis was carried out to analyze the correlation between Pak1 gene expression and clinical significance in these breast cancer patients. Results The mRNA expressions of Pak1 gene were detected in 35 cases of breast carcinoma,20 cases of benign mammary lesions and 15 cases of normal breast tissue by fluorescent real-time RT-PCR relative expression level of Pak1 mRNA were 4. 76±0. 11,2. 66±0. 10,0. 94±0. 06(P＜0. 05) ; the relative expression level of Pak1 mRNA in breast carcinoma with ER positive and ER negative were 5. 04±0. 69,4. 59±0. 57(P＜0. 05) ; the protein expression of Pak1 was detected in 35 cases of breast carcinoma and 20 cases of benign mammary lesions by immunohistochemical assay. The over expression rate of Pak1 in breast carcinoma with ER positive and ER negative were 90. 48％ (19/21) and 57. 14％ (8/14,P＜0. 05) ; in breast carcinoma with PR positive and PR negative wcre 86. 96％(20/22) and 53. 85％ (7/13,P＜0. 05) ; neither mRNA nor protein expression of Pak1 gene was correlated with age of patients, size of tumor,the level of CerbB-2(P＞0. 05) ; Conclusion Pak1 over expression is a common phenomenon in breast cancer,and may be related to the level of ER and PR of breast carcinoma.关键词
乳腺肿瘤/逆转录聚合酶链反应/免疫组织化学/p21活化激酶类引用本文复制引用
李秋云,施俊义,盛湲..Pak1基因在乳腺癌组织中的表达及临床意义[J].重庆医学,2011,40(7):646-649,后插3,5.
