山东医药2011，Vol.51Issue(4)：16-17,20,3.
p38MAPK介导Ghrelin对TNF-α诱导的人脑微血管内皮细胞MCP-1 mRNA表达的抑制
Ghrelin inhibit MCP-1 mRNA expression in human brain microvascular endothelial cells induced by tumor necrosis factor-α via p38MAPK
摘要
Abstract
Objective To investigate the effect of Ghrelin on monocyte chemoattractant protein-1 ( MCP-1) expression in human brain microvascular endothelial cells ( HBMEC) induced by TNF-α and the effects of p-38 MAPK.Methods After being cultured, different concentration TNF-α were used to treat the HBMEC.In Ghrelin pretreatment group, Ghrelin preprocessing (10 ng/ml) was performed an hour before adding the TNF-α (1.0 ng/ml).The expression of HBMEC MCP-1 mRNA was detected by RT-PCR, and the p-p38 expression was investigated by western blot.Results The expression of MCP-1 mRNA in HBMEC was increased when cells were exposed to different concentration of TNF-α with concentration dependent ( P＜ 0.05 ) , and the p-p38 expression in cytoplasm increased significantly ( P ＜0.05) .The expression of MCP-1 mRNA and p-p38 decreased significantly after the Ghrelin preprocessing ( P ＜ 0.05 ) . Conclusion Ghrelin might inhibit the MCP-1 expression in HBMEC via p38 MAPK.关键词
单核细胞趋化蛋白1/人脑微血管内皮细胞/Ghrelin分类
医药卫生引用本文复制引用
郭闻师,丁丽颖,单海燕,李健..p38MAPK介导Ghrelin对TNF-α诱导的人脑微血管内皮细胞MCP-1 mRNA表达的抑制[J].山东医药,2011,51(4):16-17,20,3.基金项目
中华医学会临床医学科研专项资金资助(09010530208). （09010530208）
