林业科学2011，Vol.47Issue(1)：42-49,8.
欧美杨R2R3-MYB家族新基因PeMYBF1的克隆及表达
Characterization and Expression of PeMYBF1 Encoding a R2R3-MYB Transcription Factor from Populus euramericana
摘要
Abstract
A MYB gene, PeMYBF1, was isolated from male inflorescence of Populus euramericana for the first time by a homologous cloning technique. Sequence analysis showed that the gene encoding protein PeMYBF1 contained only two tandem repeats of 53 amino acids in its N-terminal, which was characteristic of R2R3-MYB transcription factor, suggesting PeMYBF1 is a putative member of R2R3-MYB gene family in P. euramericana. Phylogenetic analysis based on the amino acid alignment of PeMYBF1 and other MYB proteins revealed that PeMYBF1 belonged to subgroup 19, in which members have been known to play important roles in inflorescence development. Organ-specific expression analysis revealed that PeMYBF1 was predominantly expressed in both male and female inflorescence. Our results suggested PeMYBF1 would be involved in the regulation of flower development in P. euramericana. Moreover, temporal expression pattern of PeMYBF1 revealed that PeMYBF1 expression was tightly regulated during inflorescence development.关键词
花发育/欧美杨/R2R3-MYB geneKey words
flower development/ Populus euramericana/ R2R3-MYB gene分类
农业科技引用本文复制引用
宿红艳,王磊,王仲礼,朱路英,孔冬瑞..欧美杨R2R3-MYB家族新基因PeMYBF1的克隆及表达[J].林业科学,2011,47(1):42-49,8.基金项目
the Natural Science Foundation of China (31000307),the Outstanding Young Scientist Foundation of Shandong Province (BS2010NY004),the Open Project of State Key Laboratory of Crop Biology of Shandong Agricultural University(2010KF03),and the Strengthening Project of Ludong University Subject Building. （31000307）
