法医学杂志2011，Vol.27Issue(1)：25-29,5.DOI:10.3969/j.issn.1004-5619.2011.01.007
超高效液相色谱-串联质谱法同时测定尿液中16种抗生素
Simultaneous Determination of Sixteen Antibiotics in Human Urine with Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
陈聪 1严慧 2沈保华 2卓先义2
作者信息
- 1. 复旦大学,上海医学院法医学系,上海,200032
- 2. 司法部司法鉴定科学技术研究所,上海市法医学重点实验室,上海,200063
- 折叠
摘要
Abstract
Objective To develop a method for simultaneous determination of sixteen antibiotics in human urine by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods With Piperacillin as an internal standard, the target antibiotics in urine samples were enriched and purified by Oasis HLB solid phase extraction(SPE) cartridges, then separated in a ZORBAX SB-C18 column with a gradient elution of mobile phase of 0.1％ formic acid water and acetonitrile, finally analyzed with multiple reaction monitoring (MRM) mode. Results The limits of detection (LOD) for these sixteen antibiotics were in the range of 0.05-10.0ng/mL and the limits of quantification (LOQ) in the range of 0.25-20.0ng/mL.Within the related linear range, the related coefficient (r) of sixteen antibiotics were all more than 0.995.Accuracies for these antibiotics were ranged from 82.0％-119.3％, the within-day precision were less than 13.9％. Conclusion The developed method is sensitive, specific and appropriate for the analysis of antibiotics in forensic toxicology and therapeutic drugs monitoring.关键词
法医毒理学/抗生素/尿/超高效液相色谱-串联质谱法Key words
forensic toxicology/ antibiotics/ urine/ ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)分类
社会科学引用本文复制引用
陈聪,严慧,沈保华,卓先义..超高效液相色谱-串联质谱法同时测定尿液中16种抗生素[J].法医学杂志,2011,27(1):25-29,5.
