中国美容医学2011，Vol.20Issue(2)：260-263,4.
联合运用冻融法和化学法去细胞同种异体神经修复兔面神经缺损
Repair of facial nerve defect with freeze-thaw and chemical acellular allogenic nerve in rabbits
摘要
Abstract
Objective Through improve the preparative method of extracted acelluar nerve allograft,explore an new effective alternative material for the repair of facial nerve defect.Methods Took 24 rabbits and a 1.0 cm facial nerve gap was made on the left buccal branch of the rabbits.According to different repair methods, the twenty-four rabbits were randomly divided into trial group that repaired facial nerve defect with freeze-thaw and chemical acellular allogenic nerve graft and control group that repaired facial nerve defect with inverted autologous facial nerve.Each group had 12 rabbits.Some examinations of the two groups were done on the 3th month postoperative, including visual observation,neural electrophysiological testing, image analysis of myelinated nerve fibers as well as electron microscope observation.Using t tests to comprehensive evaluation the data of the recovery of facial nerve with SPSS17.0 software package.Results Two groups of rabbits were alive, the incision healed well and the facial shape of rabbits were basic symmetry.In the trial and control groups, the conduction velocity of buccal branch was (55.74 ±10.56)m/s and(61.34 ±9.72)m/s and there was no significant difference in both conditions (P＞0.05), the quantity of myelinated nerve fibers that distanced grafts distal anastomotic 4.0mm was (18173.62±918.38)n/mm2 and (18601.21 ±982.31)n/mm2 and there was no significant difference in both conditions (P＞0.05) too.At the same time, the results of the two groups were also generally similar on electron microscope observation of the neighboring distal nerve of nerve graft.Conclusion Freeze-thaw and chemical acellular allogenic nerve allograft can satisfactory repair a certain length of facial nerve defects, and this is an effective substitute for autologous nerve graft in the repair of facial nerve defects.关键词
面神经/去细胞/神经移植/神经缺损/兔分类
生物科学引用本文复制引用
刘国栋,张荣明,崔超,张林..联合运用冻融法和化学法去细胞同种异体神经修复兔面神经缺损[J].中国美容医学,2011,20(2):260-263,4.基金项目
辽宁省科学技术厅,辽宁省科学技术计划重大、重点项目(项目编号:2009225010-43) （项目编号:2009225010-43）
