神经损伤与功能重建2011，Vol.6Issue(1)：15-18,4.DOI:10.3870/sjsscj.2011.01.004
缺血对体外培养星形胶质细胞凋亡及细胞周期的影响
Effects of Ischemia on Apoptosis and Cell Cycles of Cultured Astrocytes
余勇飞 1许康 1李平 1谢敏杰 2喻志源 2朱舟 2王伟2
作者信息
- 1. 湖北省新华医院神经内科,武汉,430015
- 2. 华中科技大学同济医学院附属同济医院神经科,武汉,430030
- 折叠
摘要
Abstract
Objective:To study the effects of ischemia on apoptosis and cell cycle of cultured astrocytes. Methods: Cell suspension was obtained by dissociating the cerebral cortex of neonatal rats. Astrocytes were purified by culture, detachment and passages. Astrocytes were treated with in vitro ischemic condition (deprivation of oxygen and serum, low glucose), harvested from ischemia-treated cultures at 3, 6, 12 and 24 h, and then stained with PI (Propidine Iodide). Contents of DNA in each sample were measured by flowcytometry. The percentage of apoptotic ceils and the cell cycle were quantified. Results: Control and ischemia groups showed no significant differences in the percentage of apoptosis cells. No apoptotic astrocytes was found throughout the course of ischemia. The percentages of S and G2/M phases in astrocytes were increased significantly at 3, 6 and 12 h after ischemia when compared with the controls, which peaked at 6 h.Conclusion: Astrocytes are relatively resistant to ischemia. Astrocytes become reactive under ischemia.关键词
缺血/星形胶质细胞/凋亡/细胞周期Key words
ischemia/ astrocyte/ apoptosis/ cell cycle分类
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余勇飞,许康,李平,谢敏杰,喻志源,朱舟,王伟..缺血对体外培养星形胶质细胞凋亡及细胞周期的影响 [J].神经损伤与功能重建,2011,6(1):15-18,4.
