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猪病毒病抗体及病原的检测及分析

段正赢 田永祥 杨克礼 梁望旺 刘泽文 邓均华 熊忠良 郭锐 周丹娜 徐涤平

安徽农业科学2011,Vol.39Issue(3):1461-1462,2.
安徽农业科学2011,Vol.39Issue(3):1461-1462,2.

猪病毒病抗体及病原的检测及分析

Detection and Analysis of Swine Virus Antibodies and Pathogen

段正赢 1田永祥 1杨克礼 1梁望旺 1刘泽文 1邓均华 1熊忠良 1郭锐 1周丹娜 1徐涤平1

作者信息

  • 1. 湖北省农业科学院畜牧兽医研究所,湖北武汉,430209
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摘要

Abstract

[ Objective] To investigate the antibody levels of swine viral disease and provide theoretical basis for disease control. [ Method ] Blood and tissue samples were collected. The antibodies and pathogens of classical swine fever ( CSF), pseudorabies ( PR ), porcine reproductive and respiratory syndrome (PRRS) and porcine circovirus disease (PCV-2) were detected by enzyme-linked immunosorbent assay (ELISA), polymerase chain reaction (PCR) and reverse transcription polymerase chain reaction (RT- PCR) method. [ Result] The results show that there were CSF and PR wild virus infections in pig farms; PRRS antibody level was very low, and the positive rate was 3.3% -17.3%; the infection rate of PCV-2 was 0 - 9.6%. [ Conclusion ] We should develop a comprehensive prevention and control measures to control diseases.

关键词

猪瘟/猪伪狂犬病/猪繁殖与呼吸综合征/猪圆环病毒病/检测分析

Key words

Classical swine fever/ Pseudorabies/ Porcine reproductive and respiratory syndrome/Porcine circovirus-2 disease/Detection and analysis

分类

农业科技

引用本文复制引用

段正赢,田永祥,杨克礼,梁望旺,刘泽文,邓均华,熊忠良,郭锐,周丹娜,徐涤平..猪病毒病抗体及病原的检测及分析[J].安徽农业科学,2011,39(3):1461-1462,2.

基金项目

"十一五"国家科技支撑计划(2006BAD06A18-6) (2006BAD06A18-6)

动物胚胎工程及分子育种湖北省重点实验室开放课题(2010ZD150~159)项目资助. (2010ZD150~159)

安徽农业科学

0517-6611

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