中国免疫学杂志2011，Vol.27Issue(4)：342-346,5.DOI:10.3969/j.issn.1000-484X.2011.04.013
β-榄香烯对EAE小鼠视神经炎治疗作用的研究
The effect on the optic neuritis of EAE mice treated by β-elemene
摘要
Abstract
Objective :To study the neuroprotective effect of β-elemene on optic neuritis of EAE mice ,and to identify how β-elemene regulates the differentiation of T helper cell subsets and the production of their specific cytokines to implement the neurprotective effect .Methods :The neuroprotective effect of β-elemene on demyelinated optic neuritis of EAE mice was observed through the behavior and morphology method (hematoxylin-eosin staining ,electromicroscope observation ) .The mRNA and protein expressions of specific cytokines and their transcript factors were observed by ELISA and real time PCR with T helper cell subsets of the EAE mice as the samples .Therapeutic effect of β-elemene was therefore evaluated .Results :β-elemene can improve clinical score and reduce axonal and myelin damage in EAE mice .β-elemene can reduce the expression of cytokine IL-17 and the transcript factor ROR γt on 11 days after immunization ,and at the same time increased the expression of Foxp3 .β-elemene also downregulated the expression of cytokine IFN-γ and its transcript factor T-bet on 19 days after immunization ,and the drug kept the expression of Foxp3 mRNA higher during the period .Conclusion :β-elemene can improve inflammatory injury of op tic neuritis in EAE mice ,and keep immune homeostasis by controlling the differentiation of T helper cell subsets .关键词
β-榄香烯/视神经炎/T淋巴细胞/白细胞介素17/干扰素γ分类
医药卫生引用本文复制引用
张荣伟,周志可,禹红梅,张惠敏,陈蕾..β-榄香烯对EAE小鼠视神经炎治疗作用的研究[J].中国免疫学杂志,2011,27(4):342-346,5.基金项目
本文为辽宁省教育厅资助项目(2009A733) （2009A733）
