四川师范大学学报（自然科学版）2011，Vol.34Issue(1)：105-109,5.DOI:10.3969/j.issn.1001-8395.2011.01.022
高效降解秸秆纤维素菌株的筛选鉴定及产酶条件优化
Screening, Identification and Optimization of Fermentation Conditions for a High Efficiency Straw- degradation Strain
摘要
Abstract
A high-efficient cellulose-degradation stain was screened from the soil of rotten straw.The selected strain (YA-14) was identified as Aspergillus fumigatus according to the 18S rRNA gene sequencing and phylogenetic analysis.Single factor experiment and orthogonal test were designed to optimize the fermentation conditions of cellulase production.The results showed that the optimum conditions of enzyme production were 2: 1 of C/N, pH = 5.0 at the beginning of the culture, 0.4 mg/mL of Mg2 + concentration, and 1.0％ of inoculation.In the optimizing conditions, after 72 h cultivation, the cellulase activity of the strain was 0.486 IU/mL, being 2.68-t(mes as against 0.181 IU/mL before the optimization.The results of solid- state fermentation showed that this strain had a high efficiency of straw cellulose degradation.关键词
秸秆/烟曲霉/纤维素降解/产酶条件优化/固体发酵Key words
straw/ Aspergillus fumigatus/ cellulose-degradation/ optimization of fermentation conditions/ solid-state fermentation分类
生物科学引用本文复制引用
王晓林,张西玉,白方文,王魁荣,陈晓敏,白林含..高效降解秸秆纤维素菌株的筛选鉴定及产酶条件优化[J].四川师范大学学报（自然科学版）,2011,34(1):105-109,5.基金项目
科技部支撑计划"秸秆植生带制造技术与产业化示范"(2007BAD39B04)资助项目 （2007BAD39B04）
