中国药理学通报2011，Vol.27Issue(1)：19-23,5.
沙蟾毒精抑制肝癌HepG2细胞黏附、迁移和侵袭的作用
Inhibitory effect of Arenobufagin on the adhesion, invasion and migration of human hepatoma carcinoma cells
摘要
Abstract
Aim To investigate inhibitory effects of Arenobufagin on the adhesion, invasion and migration of hepatoma carcinoma HepG2 cells.Methods The viability of HepG2 cells was measured by MTT assay.Cell adhesion assay, cell scratch assay and Transwell chamber assay were used to determine changes in adhesion, invasion and migration of HepG2 cells after Arenobufagin treatment.Western blot was used to detect the expression of matrix metalloproteinase 2 and 9 ( MMP 2 and MMP 9 ).Results Arenobufagin could obviously suppress the adhesive,invasive and migrative ability of HepG2 cells in a dose-dependent manner.Furthermore , it was also observed that the expression of MMP 2 and MMP 9 was decreased in HepG2 cells after arenobufagin treatment.Conclusion Arenobufagin can inhibit the adhesion, invasion and migration of HepG2 cells , which may be associated with down-regulation of the expression of MMP 2 and MMP 9.关键词
沙蟾毒精/黏附/迁移/侵袭/基质金属蛋白酶/HepG2细胞分类
医药卫生引用本文复制引用
曹惠慧,张冬梅,刘俊珊,侯春英,栗原博,叶文才..沙蟾毒精抑制肝癌HepG2细胞黏附、迁移和侵袭的作用[J].中国药理学通报,2011,27(1):19-23,5.基金项目
国家杰出青年科学基金资助项目(No 30625039) （No 30625039）
国家自然科学基金资助项目(No 90913020) （No 90913020）
广东省自然科学基金资助项目(No 9451063201002969) （No 9451063201002969）
