临床肝胆病杂志2011，Vol.27Issue(1)：49-52,4.
丙型肝炎病毒NS5A在肝癌细胞对TLR4表达的影响
Hepatitis C virus NS5A protein stimulates TLR4 expression
摘要
Abstract
Objective To investigate whether the nonstructural protein 5A (NS5A) encoded by the human hepatitis C virus RNA genome could affect the expression of TLR4.Methods HCV NS5A expression plasmid(pCN5A) was transfected into QSG7701 cells using a lipofectin reagent and the HCV NS5A protein was detected using immuno-histochemistry staining.TLR4 mRNA was evaluated by reverse transcriptase polymerase chain reaction(RT-PCR).Results HCV NS5A was detected in the cytoplasm of the QSG7701 cells transfected with pCN5A,the expression of TLR4 mRNA and protein were both up-regulated in the presence of the HCV NS5A protein.Conclusion HCV NS5A stimulates the transcription of TLR4 mRNA and the expression of TLR4 protein.关键词
肝炎病毒属/病毒非结构蛋白质类/Toll样受体 4Key words
hepacivirus/ viral nonstructural proteins/ Toll-like receptor 4分类
医药卫生引用本文复制引用
闻名,肖新强,龚国忠..丙型肝炎病毒NS5A在肝癌细胞对TLR4表达的影响[J].临床肝胆病杂志,2011,27(1):49-52,4.基金项目
国家自然科学基金(30471531) （30471531）
国家传染病"十一五"重大专项(2008ZX10002-013) （2008ZX10002-013）
