生物信息学2011，Vol.9Issue(1)：50-53,59,5.DOI:10.3969/j.issn.1672-5565.2011.01.013
猪精浆蛋白基因PSP-Ⅰ和PSP-Ⅱ启动子序列克隆及生物信息学分析
Cloning and bioinformatics analysis of promoter sequences of Porcine PSP-Ⅰ and PSP-Ⅱ
摘要
Abstract
ln this study, we amplified 1.7kbp and 1.6kbp promoters of seminal plasma protein Ⅰ and Ⅱ ( PSP - Ⅰ and PSP - Ⅱ ) genes respectively by PCR, and the PCR products were confirmed by sequencing positive TA clones. The sequencing results was submitted to align with the sequence deposited in Genbank by DNAstar program, the results showed the identities of cloned sequences of PSP - Ⅰ and PSP - Ⅱ promoters with published sequences were 99. 8％ and 96. 3％ respectively. Further bioinformatics analysis was applied to the cloned promoters of PSP - Ⅰ and PSP- Ⅱ. Sequence alignment between promoters of PSP - Ⅰ and PSP - Ⅱ by mVista revealed that region from 0 to - 1000bp was highly conservative, and the identity of region from 0 to - 200 was l00％ ,while the conservation in the upstream of - 1000bp was low. The core promoters were located in the upstream of 200bp of transcription start site of PSP - Ⅰ and PSP - Ⅱ genes by online Promoter Scan progam. The online program (TFSEARCH) analysis revealed there were many cis elements in the region from - 1000bp to transcription start site of PSP - Ⅰ and PSP - Ⅱ genes, a lot of potential transcription factor binding sites was identified as well.关键词
生物信息学/PSP-Ⅰ/PSP-Ⅱ/启动子分类
生物科学引用本文复制引用
周辉云,李庆平,吴晗,何庆玲,宋成义,陈国宏..猪精浆蛋白基因PSP-Ⅰ和PSP-Ⅱ启动子序列克隆及生物信息学分析[J].生物信息学,2011,9(1):50-53,59,5.基金项目
农业部转基因生物新品种培育重大专项 (2009ZX08010-019B和2008ZX08006-005). （2009ZX08010-019B和2008ZX08006-005）
