中国感染控制杂志2011，Vol.10Issue(1)：9-14,35,7.
口腔综合治疗台水路生物膜观察与消毒干预
Biofilms in dental unit waterlines and disinfection intervention
摘要
Abstract
Objective To evaluate the difference in the configuration and quantity of biofilms in dental unit waterlines (DUWL) before and after disinfected with sodium hypochlorite solution. Methods The samples of biofilms from DUWL supplied with 10 separate water sources were collected and the microorganisms were quantified after incubated for 48h, and the configuration of biofilms were observed with confocal laser scanning microscope (CLSM)and scanning electronic microscope (SEM). The sodium hypochlorite at concentration of 525 mg/L was used for disinfection of DUWL. Results The number of bacteria in biofilms at the baseline was 1.7× 103 CFU/cm2 before disinfection and 0 CFU/cm2 after disinfection with sodium hypochlorite solution, there was significant difference between the two (t = 12. 03, P = 0. 02) . CLSM and SEM showed that before disinfection, biofilm existed in the inner wall of DUWL,and bacilli and cocci distributed in the matrix; after disinfection, the configuration of biofilms, particularly fence-like structures were destroyed, but matrix was still existed. Conclusion DUWL has been contaminated heavily with a number of microbes, and the inner walls of DUWL is covered with biofilm, which is composed of millions of bacteria. The high concentration of microbes in DUWL is bringing the high risk of cross infection to both dental professionals and patients. Sodium hypochlorite at the concentration of 525 mg/L may be used as a disinfection solution for DUWL with separate water source.关键词
口腔综合治疗台水路/生物膜/条件致病菌/次氯酸钠/消毒/感染控制/医院感染分类
医药卫生引用本文复制引用
章小缓,凌均棨,姬亚昆,侯水平,陈守义..口腔综合治疗台水路生物膜观察与消毒干预[J].中国感染控制杂志,2011,10(1):9-14,35,7.基金项目
广东省医学科研基金项目(A2008229) （A2008229）
