中国兽医科学2011，Vol.41Issue(1)：31-35,5.
猪流行性腹泻病毒CH/S株M蛋白全长的原核表达及其反应原性分析
Expression of the whole M protein of porcine epidemic diarrhea virus CH/S strain in Escherichia coli and analysis of its reactogenicity
摘要
Abstract
One pair of primers was designed according to the published sequences of M gene of porcine epidemic diarrhea virus(PEDV) CH/S strain. The M gene was amplified with the pair of primers by RTPCR and cloned into the prokaryotic expression vector pMXB10 to construct a recombinant plasmid pMXBM. After sequencing, the recombinant was transformed into Escherichia coli BL21 (DE3) competent cells.The transformed bacteria induced by IPTG produced a fusion protein(MBP-M-CBD) of 95 ku. In result,inducing with 0.8 mmol/L IPTG for 6 h was the best conditions for expression of M protein. The recombinant protein expressed in the bacteria was in the form of inclusion bodies. The recombinant protein could react with the rabbit anti-PEDV serum and the monoclonal antibody against M protein of PEDV in Western-blot tests,indicating that the expressed protein possessed reactogenicity. The result laid the foundation for further studies of PEDV M protein.关键词
猪流行性腹泻病毒/M蛋白/原核表达/免疫印迹分类
农业科技引用本文复制引用
张志榜,陈建飞,时洪艳,陈小金,冯力..猪流行性腹泻病毒CH/S株M蛋白全长的原核表达及其反应原性分析[J].中国兽医科学,2011,41(1):31-35,5.基金项目
国家"十一五"科技支撑计划项目(2006BAD06A07) （2006BAD06A07）
