大豆科学2011，Vol.30Issue(1)：20-23,4.
大豆成熟种子胚尖基因枪法转化体系的优化
Optimization of Biolistics Transformation of Embryonic Tips of Soybean [ Glycine max (L.) Merrill] Mature Seeds
摘要
Abstract
To establish an efficient transformation system of embryonic tips of soybean mature seeds, effects of 6-BA and hygromycin B concentration were studied during biolistics transformation of soybean [ Glycine max (L.) Merrill cv ‘ Hedou12’ ]. The UidA (GUS) gene was employed as a reporter gene to reveal the foreign gene expression and integration.Both number and elongation length of multiple shoot reached the highest values at 5 mg· L-1 6-BA for 16 h induction of embryonic tips of mature seeds. The optimal selection scheme of transformants was a gradient concentration of hygromycin B from 5 mg· L-1 to 15 mg· L-1. The expression of GUS gene was observed in both embryonic tips after particle bombardment and young leaves of progeny transformants. The integration of GUS gene into the soybean genome was demonstrated by PCB and sequencing analysis.关键词
大豆/成熟种子胚尖/基因枪法/遗传转化Key words
Glycine max/ Embryonic tips of mature seeds/ Biolistics/ Genetic transformation分类
农业科技引用本文复制引用
叶美,张敏,杨素欣,邵群,安海龙,曹子谊,冯献忠..大豆成熟种子胚尖基因枪法转化体系的优化[J].大豆科学,2011,30(1):20-23,4.基金项目
国家转基因重大专项资助项目(2009ZX08009-026B) （2009ZX08009-026B）
山东省自然科学杰出青年基金资助项目(JQ200909). （JQ200909）
