基础医学与临床2011，Vol.31Issue(1)：25-31,7.
福辛普利和缬沙坦抑制Ang Ⅱ诱导的大鼠肾小管上皮细胞klotho基因表达下调
Role of fosinopril and valsartan on inhibiting klotho gene down-expression induced by Ang Ⅱ
摘要
Abstract
Objective To investigate role of fosinopril (Fos) and Valsartan (Val) on klotho gene expression induced by Ang Ⅱ and the relationship between klotho and TGF-β1.Methods Culture cells, NRK-52E, were incubated with Ang Ⅱ, Fos and Val.Five groups, control, Ang Ⅱ ( 10-7mol/L), Ang Ⅱ (10-7mol/L) + Fos (10-5mol/L), Ang Ⅱ (10-7mol/L) + Val(10-5mol/L) and Ang Ⅱ (10-7mol/L) + Fos(10-5mol/L) + Val (10-5mol/L) ,were divided to observe the expression of the klotho and TGF-β1 mRNA and protein.The klotho and TGF-β1 mRNA were detected by reverse transcription-polymerase chain reaction (RT-PCR) ,immunohistochemistry (IHC) and Western blot was applied to determine the klotho and TGF-β1 protein expression.The linear relation ship between klotho and TGF - β 1 protein expression was analyzed by Pearson's.Results ( 1 ) Ang Ⅱ induced an up-regulated expression ot TGF-β1 mRNA, and associated with down-regulated klotho mRNA in NRK-52E as compared to control ( all P ＜ 0.05 ); in cells incubated with Fos or Valor booth of all, the klotho mRNA expression was enheanced, and the TGF-β1 mRNA expression was inhibited; combined Fos and Valcould not enhance the expression of klotho gene, no significant different was shown as compared to the Fos group and Valgroup.(2)IHC and Western blot revealed that klotho protein was increased, TGF-β1 protein was decreased in Fos group, Val group and combination group as compared to Ang Ⅱ group, but no significantly different among the three groups was shown as compared to the Val group or Fos group.(3)Negative relationship was showed between klotho and TGF-β1 protein expression(r = -0.717, P ＜ 0.05 ).Conclusion Fosinopril and valsartan can enhance the expression of klotho gene, and down-regulate TGF-β1 expression in NRK-52E, the increased level of klotho expression may be one of the important factor for preventing the renal fibrosis induced by TGF-β1.关键词
福辛普利/缬沙坦/klotho基因/生长转换因子-β1Key words
fosinopril/ valsartan/ klotho gene/ TGF-β1分类
医药卫生引用本文复制引用
林书典,周巧玲,詹锋..福辛普利和缬沙坦抑制Ang Ⅱ诱导的大鼠肾小管上皮细胞klotho基因表达下调[J].基础医学与临床,2011,31(1):25-31,7.基金项目
海南省自然科学基金(309087) （309087）
